研究成果
发表论文:
1. Zhengying You, Xiaogang Ye, Lupeng Ye, Qiujie Qian, Meiyu Wu, Jia Song, Jiaqian Che, Boxiong Zhong*. Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin. Scientific Reports, 2018 29; 8(1):15956.
2. Qiujie Qian#, Zhengying You# (Co-first author), Lupeng Ye, Jiaqian Che,Yiran Wang, Shaohua Wang, Boxiong Zhong*. High-efficiency production of human serum albumin in the posterior silk glands of transgenic silkworms, Bombyx mori L. PloS One, 2018, 13(1): e0191507.
3. Zhengying You#, Qiujie Qian#, Yiran Wang, Jiaqian Che, Lupeng Ye, Lirong Shen*, Boxiong Zhong* Transgenic silkworms secrete the recombinant glycosylated MRJP1 protein of Chinese honeybee, Apis cerana cerana. Transgenic Res, 2017.11. doi: 10.1007/s11248-017-0034-1.
4. Shaohua Wang#, Zhengying You# (Co-first author), Mao Feng, Jiaqian Che, Yuyu Zhang, Qiujie Qian, Setsuko Komatsu*, Bo-xiong Zhong*. Analyses of the molecular mechanisms associated with silk production in silkworm by iTRAQ-based proteomics and RNA-sequencing-based transcriptomics. Journal of proteome research, 2016 15(1):15-28.
5. Jianying Li, Fang Cai, Xiaogang Ye, Jianshe Liang, Jianke Li, Meiyu Wu, Dan Zhao, Zhendong Jiang, Zhengying You, and Boxiong Zhong* Comparative Proteomic Analysis of Posterior Silk Glands of Wild and Domesticated Silkworms Reveals Functional Evolution during Domestication. Journal of proteome research, 2017 16(7):2495-2507.
6. Jia Song, Jiaqian Che, Zhengying You, Xiaogang Ye, Jisheng Li, Lupeng Ye, Yuyu Zhang, Qiujie Qian, Zhong Boxiong*. The clustered regularly interspaced short palindromic repeats/associated proteins system for the induction of gene mutations and phenotypic changes in Bombyx mori. Acta Biochimica Et Biophysica. 2016 48(12):1112-1119.
7. Jia Song, Jiaqian Che, Zhengying You, Lupeng Ye, Jisheng Li, Yuyu Zhang, Qiujie Qian, Boxiong Zhong*. Phosphoproteomic analysis of the posterior silk gland of Bombyx mori provides novel insight into phosphorylation regulating the silk production. Journal of Proteomics, 2016 148:194-201.
8. Lupeng Ye, Zhengying You, Qiujie Qian, Yuyu Zhang, Jiaqian Che, Jia Song, Boxiong Zhong*. TAL effectors mediate high-efficiency transposition of the piggyBac transposon in silkworm Bombyx mori L. Scientific Reports, 2015, 5:17172.
9. Lupeng Ye, Qiujie Qian, Yuyu Zhang, Zhengying You, Jiaqian Che, Jia Song, Boxiong Zhong*. Analysis of the sericin1 promoter and assisted detection of exogenous gene expression efficiency in the silkworm Bombyx mori L. Scientific Reports, 2015, 5:8301
10. Shaohua Wang, Zhengying You, Lupeng Ye, Jiaqian Che, Qiujie Qian, Yohei Nanjo, Setsuko Komatsu, and Boxiong Zhong*. Quantitative Proteomic and Transcriptomic Analyses of Molecular Mechanisms Associated with Low Silk Production in Silkworm Bombyx mori. Journal of proteome research, 2014 13(2):735-751.
11. Jisheng Li, Yimei Cai, Lupeng Ye, Shaohua Wang, Jiaqian Che, Zhengying You, Jun Yu and Boxiong Zhong*. MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori. BMC Genomics, 2014 15:410.
12. Jine Chen, Jianying Li, Zhengying You, Lili Liu, Jianshe Liang, Yingying Ma, Ming Chen, Huarong Zhang, Zhendong Jiang, and Bo-xiong Zhong*. Proteome Analysis of Silkworm, Bombyx mori Larval Gonads: Characterization of Proteins Involved in Sexual Dimorphism and Gametogenesis. Journal of proteome research, 2013 12(6):2422-38.
13. Jisheng Li, Wei Fan, Zhengying You, Boxiong Zhong*. Bioinformatics analysis on structural features of microRNA precursors in insects. European Journal of Entomology. 2013, 110(1):13-20.
14. Lupeng Ye, Lanfang Zhuang, Jisheng Li, Zhengying You, Jianshe Liang, Hao Wei, Jianrong Lin,and Boxiong Zhong*. Analysis of the activity of virus internal ribosome entry site in silkworm Bombyx mori. Acta Biochimica Et Biophysica. 2013 45(7):534-539.
15. Ling Lin, Liu Xuelan, Xu Jiaping*, You Zhengying, Zhou Jingbo. Silkworm eggs: An ideal model for studying the biological effects of low energy Ar+ ion interaction in animals. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2011, 269:1997-2000.
16. 尤征英，危浩，阮系真，叶露鹏，王少华，周继勇，钟伯雄*. 家蚕丝腺生物反应器表达狂犬病病毒核蛋白.中国农业科学. 2013, 46 (18).
17. 尤征英, 周武松, 徐家萍*, 杨莹, 凌琳, 王文兵. 家蚕 Bms3a基因真核表达载体的构建及其在家蚕细胞和蚕体中的表达. 激光生物学报. 2011, 20 (2):39-45.
申请专利：
1. 钟伯雄， 尤征英， 叶露鹏， 钱秋杰。利用家蚕丝腺生物反应器合成分泌黑寡妇蜘蛛牵引丝蛋白1的方法。
2. 钟伯雄， 尤征英， 叶露鹏， 钱秋杰。利用家蚕丝腺生物反应器合成分泌黑寡妇蜘蛛牵引丝蛋白2的方法。
3. 钟伯雄，叶露鹏，钱秋杰，张玉玉，尤征英，王少华，车家倩，宋佳。用标记基因预测外源基因表达量及筛选转基因生物的方法。
4. 钟伯雄，叶露鹏，钱秋杰，尤征英，车家倩，张玉玉，宋佳。类转录激活子效应蛋白介导的高效转基因方法。
5. 钟伯雄，钱秋杰，叶露鹏，尤征英，车家倩，等。家蚕后部丝腺合成分泌人血清白蛋白的方法。
主持和参与的科研项目：
1． 家蚕合成分泌优异家蚕/蜘蛛丝复合丝纤维的研究，国家自然科学基金青年项目， 31702183，22万，2018.01 - 2020.12. 在研，主持
2． 探索家蚕丝蛋白基因高效表达调控机理的研究，浙江理工大学科研启动项目，11612932618138，15万元2018.06-2023.06. 在研，主持
3. 转蜘蛛牵引丝蛋白蚕丝优异性能的分子机理的探索，中国博士后科学基金，编号2016M591989, 5万元，2016.05-2017.10. 结题，主持
4. 蛋白泛素化修饰异常与家蚕裸蛹的关系研究，国家自然科学基金，在研，2018.01-2021.12，. 参与
