主要科研项目

1. 浙江省公益技术研究计划项目 ，放线菌细胞色素P450酶的生物转化、催化机制及其应用研究，2020-01至2022-12，10万元，主持。

2. 国家自然科学基金青年项目，细胞色素P450介导的二氢黄酮生物合成及羟基化催化机制研究，2015-01-01至2017-12-31，23万元，主持。

3. 浙江大学科研青年专项，链霉菌Streptomyces avermitilis细胞色素P450酶的结构与功能研究，2013-01至2014-12，25万元，主持。

4. 国家自然科学基金面上项目，基于Aplysiatoxin衍生物的钾离子通道Kv1.5抑制剂的发现及作用机制研究，2020-01-01至2023-12-31，66万元，主参。

5. 国家自然科学基金面上项目，利用分子网络技术和两种新胁迫方法发现隐蔽海洋微生物天然产物，2019-01至2022-12，62万元，主参。

6. 国家自然科学基金青年项目，海洋曲霉属真菌次级代谢产物生物合成基因簇的异源表达研究，2015-01-01至2017-12-31，27万元，主参。

7. 国家自然科学基金青年项目，食物链对腹泻性贝类毒素产毒藻鳍藻生长和产毒的调控研究，2014-01-01至2016-12-31，27万元，主参。

8.  浙江省自然科学基金杰出青年科学基金项目，微生物药物生物合成机器的解析与重塑，2019-01至2022-01，80万元，主参。

9.  浙江省科技厅项目，浙江沿海腹泻性贝类毒素爆发的预警预报技术， 2013-07至2015-12，15万元，主参。

代表性论文（第一或通讯作者）

1. Gao Q., Ma B., Wang Q., Zhang H., Fushinobu S., Yang J., Lin S., Sun K., Han B-N*, Xu L-H*, Improved 2α-hydroxylation efficiency of steroids by CYP154C2 using structure-guided rational design, Applied and Environmental Microbiology, 2023, In press.

2. Lin Su., Ma B., Gao Q., Yang J., Lai G., Lin R., Yang B., Han B-N, and Xu L-H. * The 16α-hydroxylation of progesterone by Cytochrome P450 107X1 from Streptomyces avermitilis. Chemistry and Biodiversity, 2022, 19 (5):

3. Ma B., Wang Q., Han B-N., Ikeda H., Zhang C.,* and Xu L-H* Hydroxylation, epoxidation, and dehydrogenation of capsaicin by a microbial promiscuous Cytochrome P450 105D7. Chemistry and Biodiversiy, 2021, 18 (4):

4. Wang Q., Ma B., Fushinobu S., Zhang C.*, Xu L-H.* Regio- and stereoselective hydroxylation of testosterone by a novel cytochrome P450 154C2 from Streptomyces avermitilis. Biochemical and Biophysical Research Communications, 2020, 522(2):355-361

5. Ma B., Wang Q., Ikeda H., Zhang C., Xu L-H.* Hydroxylation of Steroids by A Microbial Substrate-Promiscuous Cytochrome P450 105D7: Key Arginine Residues for Rational Design. Applied and Environmental Microbiology, 2019, 85 (23)

6. Xu L-H.*, Du Y-L. Rational and semi-rational engineering of cytochrome P450s for biotechnological applications. Synthetic and Systems Biotechnology, 2018, 3(4): 283-290

7. Zhang C. #, Xu L-H. #, Zhou H.,Tan Z., Xie Q., Xu Y. Biodegradation of n-hexadecane by enteric bacteria isolated from an oil-field wastewater treatment plant. Fresenius Environmental Bulletin, 2018, 27(7): 4942-4951

8. Xu L-H., Tan Z., Zhang C.*, Liu L., Liu Y., Zhang X., Wu J., Xie Q. Performance and microbial diversity of a full-scale oilfield wastewater treatment plant. Desalination and Water Treatment, 2017, 99: 239-247

9. Yao Q., Ma L., Liu L., Ikeda H., Fushinobu S., Li S., Xu L-H*. Hydroxylation of compactin (ML-236B) mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis. Journal of Microbiology and Biotechnology,2017, 27(5): 956-964

10. Liu L., Yao Q., Ma Z., Ikeda H., Fushinobu S., Xu L-H*. Hydroxylation of flavanones by Cytochrome P450 105D7 from Streptomyces avermitilis. Journal of Molecular Catalysis B: Enzymatic, 2016, 132: 91-97

11. Xu L-H*, Ikeda H., Liu L., Arakawa T., Wakagi T., Shoun H., Fushinobu S. Structural basis for the 4’-hydroxylation of diclofenac by a microbial cytochrome P450 monooxygenase. Applied Microbiology and Biotechnology, 2015, 99 (7): 3081-3091

12. Takamatsu S.#, Xu L-H.#, Fushinobu S., Shoun H., Komatsu M., Cane D.E., Ikeda H.* Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites: Hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SVA_7469) of Streptomyces avermitilis. Journal of Antibiotics, 2011, 64 (1): 65-71

13. Xu L-H., Fushinobu S., Takamatsu S., Wakagi T., Ikeda H., and Shoun H.* Regio- and stereospecificity of filipin hydroxylation sites revealed by crystal structures of cytochrome P450 105P1 and 105D6 from Streptomyces avermitilis. Journal of Biological Chemistry, 2010, 285 (22): 16844-16853

14. Xu L-H., Fushinobu S., Ikeda H., Wakagi T. and Shoun H.* Crystal structures of Cytochrome P450 105P1 from Streptomyces avermitilis: conformational flexibility and histidine-liganded state. Journal of Bacteriology, 2009, 191 (4): 1211-1219

授权专利

1. 一种生物转化类固醇类化合物获取羟基化化合物的方法，许莲花、马炳炳、王倩文，专利号：ZL2019104483530，已转化

2. 一种利用细胞色素P450酶合成2α-羟基化类固醇化合物的方法，许莲花、王倩文、马炳炳，专利号：ZL2019111191804，已转化