研究成果
主持及作为课题组核心成员申请实施项目
1、辽宁省科学事业公益基金：智能好氧-厌氧耦合调控 NADH 供给产氢关键技术研究 (No.2014003020)
2、博士后基金：产气肠杆菌氢酶基因的克隆、高效表达及其产氢过程强化与定量追踪（20060390451）
3、沈阳师范大学主任基金：产气肠杆菌氢酶相关基因克隆、表达及其产氢过程的强化（No. SY200607）
4、自然基金重点项目：智能磷酸转移酶的分子酶工程技术及其应用基础研究(Grant No. 20836004)
5、“十一五”国家高技术研究发展计划 (863): 颗粒状甲烷单加氧酶的高效表达其智能调控 (GrantNo.2006A02Z203)
6、“十一五” 国家科技支撑计划：阿维菌素基因工程法清洁生产新工艺的研究及开发 (Grant No.2 006EP805)
7、北京市科委科技攻关项目：大气压射频辉光放电等离子体消毒装置的研究(Grant No.Z07000200540705)
8、北京市自然科学基金资助项目：常压低温等离子体特性及其作为微生物育种新方法研究 (GrantNo. 3083021)
9、自然科学基金面上项目：
(1) 表面活性剂调控恶臭甲单胞杆菌胞内酶反应机理研究(Grant No. 20676071)；
(2)微米臭氧气泡强化剩余污泥溶解减量的机理研究 (Grant No. 20777045)
10、贵州省科技厅重大专项计划项目：酶法高效清洁生产低分子量肝素的工程化关键技术开发及产业化示范黔科合 GY 字(2007) -1024
研究成果
[1] Zhao H.X., Lu Y., Wang L Y., Zhang C., Xin-Hui Xing*.Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes.Bioresource Technology 2014 (Submitted).
[2] Lu Y., Zhao H X., Zhang C., Xing X H. Effect of NADH regeneration on the hydrogen production by overexpression of NAD-dependent formate dehydrogenase in different mutants of Enterobacter aerogenes International Journal of Hydrogen Energy2014 (Submitted).
[3].Ma†,K., Zhao†, H.X., Zhang, C., Lu, Y., Xing, X.H*.Impairment of NADH dehydrogenase for increased hydrogen production and its effect on metabolic flux redistribution in wild strain and mutants of Enterobacter aerogenes. International Journal of Hydrogen Energy. 2012, 37:15875-85 (†Equal contribution first author)
[4]. Zhao, H.X., Liu, X.M., Xie, B.E., Chen, S.F. Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nifB Promoter Analysis, Science in China: Series C Life Sciences. 2006, 49(2): 115-122.
[5]. Zhaoa, H.X., Maa, K., Lua, Y., Zhanga, C., Wanga, L.Y., Xing, X.H*.Cloning and knockout of formate hydrogen lyase and H2-uptake hydrogenase genes in E. aerogenes for enhanced hydrogen production.International Journal of Hydrogen Energy 2009, 34(1 ): 186-194.
[6].赵洪新，刘旭明，谢宝恩，陈三凤.Paenibacillus massiliensis T7 固氮基因簇nifBHDKENX 的克隆、序列分析及铵和氧对nifB 启动子的调控 中国科学C 辑 生命科学, 2005, 35(5):424-432.
[7]. Zhao, H.X., Yang, C., Lu, Y., Zhang, C., Xing, X.H. Characteristics of enzymatic phosphorylation of inosine by E.aerogenes IAM1083 (以产气肠杆菌催化肌苷5′-位磷酸化的特性).J T singhua U niv (清华大学学报 (自然科学版) Sci & Tech), 2008, Vo l. 48, No. 12 (In Chinese, EI Source).
[8].Zhao, H.X., Lai, Q.h., Yang. C., Zhang, Z.N, Xing, X.H*.Isolation phoC from E. aerogenes and its characteristic on enzymatic phosphorylation of inosine by fusion PhoC with MBP (产气肠杆菌磷酸酶基因克隆表达及其特性).Journal of Chemical Industry and Engineering (化工学报China).2008,159(8):2071 -2078.
[9].Liu, X.M, Zhao, H.X., Chen, S.F. Colonization of maize and rice plants by strain Bacillus megaterium C4.Current Microbiology. 2006, 52 (3): 186-190.
[10].Lu, Y., Zhao, H.X., Zhang, C., Lai Q.H., Xing, X.H*.Perturbation of formate pathway for hydrogen production by expressions of formate hydrogen lyase and its transcriptional activator in wild E.aerogenes and its mutants. International Journal of Hydrogen Energy.2009, 34(12):5072-5079.
[11].Lu, Y., Zhao, H.X., Zhang, C., Lai, Q.H., Wu, X., Xing, X.H*. Expression of NAD+-dependent formate dehydrogenase in E.aerogenes and its involvement in anaerobic metabolism and H2 production. Biotechnol Lett.2009, 31:1525-1530.
[12].Lu, Y.,Zhao, H.X., Zhang, C., Lai, Q.H., Xing, X.H*.Alteration of hydrogen metabolism ofldh-deleted E.aerogenes by over-expression of NAD (+)-dependent formate dehydrogenase. Applied Microbiology and Biotechnology, 2010, 86(1):255-262.
[13].Lu, Ya., Lai, Q.Ha., Zhang,Ca, Zhao, H.Xa., , Ma,Ka, et al. Characteristics of hydrogen and methane production from cornstalks by an augmented two-or three-stage anaerobic fermentation process.Bioresource Technology. 2009, (100):2889–2895.
[14].Lu Y, Zhang C, Zhao H X, Xing X H. Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum. Appl Biochem Biotechnol. 2012,167(4):732-42.
[15]. Wang1, L.Y., Huang1, Z.L., Li2. G., Zhao1, H.X., et al. Novel mutation breeding method for S.avermitilis using atmospheric-pressure glow discharge plasma. Applied Microbiology 2010 Mar;108(3):851 -8.
[16]. Liu†, A.R., Zhao†, L., Bai†, H., Zhao‡, H.X, et al. Polypyrrole actuator with a bioadhesive surface for accumulating bacteria from physiological media. ACS Appied Material Interfaces 2009,1(4):951–955.
[17]. Li,G., Li,H.P., Wang, L.Y., Wang, S., Zhao, H.X., et al. Genetic effects of radio-Frequency,atmospheric-pressure glow discharges with helium. Applied Physics Letters.2008, 92, 221504.
[18]. Chena, b, S., Yea, F.C., Zhao, H.Xa., Chena, Y., et al. Biochemical Analysis and Kinetic Modeling of the Thermal Inactivation of MBP-fused Heparinase I: Implications for a Comprehensive Thermostabiliztion Strategy. Biotechnol Bioeng 2011 , 108( 8): 1841–1851.
[19].Xua,F., Wanga,J.P., Chena,S.F*., Qinb,W.S., Yuc,Z.L., Zhaod, H.X., et al. Strain Improvement for Enhanced Production of Cellulase in Trichoderma viride. Appl Biochem Microbiol 2011, 47( 1 ):53-58.
[20]. Lu, Ya., Wanga, L.Y., Ma, Ka., Li,Gb., Zhang,Ca, Zhaoa