研究成果
我的研究成果
发表主要论文和著作:
1. Chen J, Shu T, Chen J, Ye M, Lv Z, Nie Z, Gai Q, Yu W, Zhang Y. Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori Protein J, 2015, 34(4):256-66.
2. Nie Z, Zhu H, Zhou Y, Wu C, Liu Y, Sheng Q, Lv Z, Zhang W, Yu W, Jiang C, Xie L, Zhang Y, Yao J. Comprehensive profiling of lysine acetylation suggests the widespread function is regulated by protein acetylation in the silkworm, Bombyx mori. Proteomics, 2015, 15(18):3253-66.
3. Li Y, Wang W, Cheng D, Wang T, Lu C, Chen J, Nie Z, Zhang W, Lv Z, Wu W, Shu J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Mar Drugs, 2015, 13(5):2955-66.
4. Zhang W, Wang J, Zhang C, Fang Q, Shu J, Li S, Jin J, Wang D, Nie Z, Lv Z, Zhang Y. Synergetic Protein Factors That Improve rhGM-CSF Absorption via an Oral Route Exist in Silkworm Pupae. Mol Pharm, 2015, 12(5):1347-55.
5. Li S, Wei Z, Chen J, Chen Y, Lv Z, Yu W, Meng Q, Jin Y. Oral administration of a fusion protein between the cholera toxin B subunit and the 42-amino acid isoform of amyloid-β peptide produced in silkworm pupae protects against Alzheimer's disease in mice. PLoS One, 2014, 9(12):e113585.
6. Chen J, Shu T, Lv Z, Nie Z, Chen J, Chen H, Yu W, Gai Q, Zhang Y. Purification and functional characterization of a protein: Bombyx mori human growth hormone like protein in silkworm pupa. PLoS One, 2014, 9(12):e114351.
7. Gong Y, Wang X, Indran IR, Zhang SJ, Lv Z, Li J, Holmes M, Tang YZ, Yong EL .Phytoplankton blooms: an overlooked marine source of natural endocrine disrupting chemicals. Ecotoxicol Environ Saf, 2014, 107:126-32.
8. Liu M, Wang C, Li D, Liu Y, Sheng Q, Lv Z, Yu W, Wang D, Zhang Y, Nie Z. Cloning and expression characteristics of the notch-associated gene BmE(spl)mγ from silkworm, Bombyx mori, Appl Biochem Biotechnol, 2014, 173(8):2065-75.
9. Liu Y, Gao Z, Guo Q, Wang T, Lu C, Chen Y, Sheng Q, Chen J, Nie Z, Zhang Y, Wu W, Lv Z and Shu J. Anti-Diabetic Effects of CTB-APSL Fusion Protein in Type 2 Diabetic Mice. Mar Drugs, 2014, 12(3), 1512-1529.
10. Lu C, Zhang J, Nie Z, Chen J, Zhang W, Ren X, Yu W, Liu L, Jiang C, Zhang Y, Guo J, Wu W, Shu J, and Lv Z. Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark, Chiloscyllium plagiosum. BioMed Research International, 2013, ID 795676, 2013.
11. Zhang J , Liu Y, Zhang X, Nie Z, Chen J,Liu L, Li J, Zhang Y , Guo J, Lv Z . Identification of MicroRNAs in Chiloscyllium plagiosum (whitespotted bamboo shark) liver by Illumina sequencing. Gene, 2013,527（1）:259-65.
12. Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Lv Z. Expression of Active Peptide from Shark Liver Gene in Silkworm, Bombyx mori. and Preliminary Research on Oral Administration of the Recombinant Protein. Mar Drugs, 2013,11(5):1492-505.
13. Lv Z， Wang T, Zhuang W，Wang D， Chen J，Nie Z，Liu L，Zhang W，Wang L， Wang D，Wu X，Li J，Qian L， and Zhang Y. The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori). International Journal of Genomics, 2013, Article ID 269609, 8 pages.
14. Lv Z, Liu Y, Chen Y, Li B, Liu L, Chen J, Nie Z, Sheng Q, Yu W, Zhang Y Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori. American Journal of Molecular Biology, 2012, 2, 224-231
15. Yang L, Lu X, Liu Y, Lv Z, Chen J, Yu W, Zhang Y, Nie Z. Expression analysis of miRNAs in BmN cells. Gene, 2012, 505(2):240-5.
16. Xu T, Wang B, Wang L, Zhang Y, Lv Z. Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity. Acta Biochim Pol, 2012, 59(2):231-6. (Corresponding author)
17. Yu W, Li J, Wang M, Quan Y, Chen J, Nie Z, Lv Z, Zhang Y. The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virol J, 2012, 9(1):90.
18. Quan Y, Liu G, Yu W, Nie Z, Chen J, Lv Z, Zhang Y. Expression, Purification, and Characterization of Ras Protein (BmRas1) from Bombyx mori. Comp Funct Genomics, 2012, 2012:747539.
19. Lv Z, Zhang X, Liu L, Chen J, Nie Z, Sheng Q, Zhang W, Jiang C, Yu W, Wang D, Wu X, Zhang S, Li J, Zhang Y. Characterization of a gene encoding prohibitin in silkworm, Bombyx mori. Gene. 2012, 502(2):118-24.
20. Zhang W, Zhang C, Lv Z, Fang D, Wang D, Nie Z, Yu W, Lan H, Jiang C, Zhang Y. Molecular characterization, tissue distribution, subcellular localization and actin-sequestering function of a thymosin protein from silkworm. PLoS One, 2012, 7(2):e31040.
21. Chen J, Gai Q, Lv Z, Chen J, Nie Z, Wu X,, Zhang Y. All-trans retinoic acid affects subcellular localization of a novel BmNIF3l pro,tein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori). Arch Insect Biochem Physiol, 2010, 74(4):217-31.
22. Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y, Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu W, Chen J, Wu X, Zhang Y.In vivo bioassay of recombinant human growth hormone synthesized in B. mori pupae. J Biomed Biotechnol, 2010, 2010:306462.
23. Wu X, Yang D, Zhu X, Feng Z, Lv Z, Zhang Y, Shen B, Xu Z. Iso-migrastatin Titer Improvement in the Engineered Streptomyces lividans SB11002 Strain by Optimization of Fermentation Conditions. Biotechnol. Bioprocess Eng, 2010, 15(4):664-669.
24. Wang M, Cai J, Huang L, Lv Z, Zhang Y, Xu Z. High-level expression and efficient purification of bioactive swollenin in Aspergillus oryzae. Appl Biochem Biotechnol, 2010, 162(7):2027-36.
25. Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the gene BmEm4, a homologue of Drosophila E(spl)m4, from the silkworm, Bombyx mori. Comp Funct Genomics, 2009, 2009:947490.
26. Nie Z, Lv Z, Qian J, Chen J, Li S, Sheng Q, Wang D, Shen H, Yu W, Wu X, Zhang Y.Molecular cloning and expression characterization of translationally controlled tumor protein in silkworm pupae. Mol Biol Rep, 2010, 37(6):2621-8.
27. Lv Z, Ou Y, Li Q, Zhang W, Ye B, Wu W. Expression, purification and bioactivities analysis of recombinant active peptide from shark liver. Mar Drugs, 2009, 7(2):258-67.
28. Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y. Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori. Comp Funct Genomics, 2009, 2009:416040.
29. Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics, 2009, 9(4):447-54.
30. Zhang W, Lv Z, Nie Z, Chen G, Chen J, Sheng Q, Yu W, Jin Y, Wu X, Zhang Y. Bioavailability of orally administered rhGM-CSF: a single-dose, randomized, open-label, two-period crossover trial. PLoS One, 2009, 4(5):e5353. (并列第一）
31. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X, Chen J, Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS One, 2008, 3(12):e3933.(并列第一）
32. Zhang Y(Corresponding author), Lv Z, Chen J, Chen Q, Quan Y, Kong L, Zhang H, Li S, Zheng Q, Chen J, Nie Z, Wang J, Jin Y, Wu X. A novel method for isolation of membrane proteins: a baculovirus surface display system.Proteomics, 2008, 8(20):4178-85.
33. Nie Z, Xu J, Chen J, Lv Z, Wang D, Sheng Q, Wu Y, Wang X, Wu X, Zhang Y. Expression analysis and characteristics of profilin gene from silkworm, Bombyx mori. Appl Biochem Biotechnol, 2009, 158(1):59-71.
34. Nie Z, Zhou R, Chen J, Wang D, Lv Z, He P, Wang X, Shen H, Wu X, Zhang Y. Subcellular localization and RNA interference of an RNA methyltransferase gene from silkworm, Bombyx mori. Comp Funct Genomics, 2008, 2008:571023.
35. He PA, Nie Z, Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y, Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC Genomics, 2008, 9:248.
36. Chen J, Chen J, Gai Q, Lv Z, Wang D, Nie Z, Wang J, Wang X, Wu X, Zhang Y. Molecular characterization and immunohis,tochemical localization of a novel troponin C during silkworm development.Cell Tissue Res, 2008, 331(3):725-38.
37. Kong L, Lv Z, Chen J, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). Biochim Biophys Acta, 2007, 1770(12):1598-604.
38. Zhang Y, Chen J, Lv Z, Nie Z, Zhang X, Wu X. Can 29kDa rhGM-CSF expressed by silkworm pupae bioreactor bring into effect as active cytokine through orally administration? Eur J Pharm Sci, 2006, 28(3):212-23.
39. Nie Z, Zhang Z, Wang D, He P, Jiang C, Song L, Chen F, Xu J, Yang L, Yu L, Chen J, Lv Z, Lu J, Wu X, Zhang Y. Complete sequence and organization of Antheraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics. 2007, 8:248.
40. Wang X, Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and functional analysis of the cellular retinoic acid binding protein from silkworm pupae (Bombyx mori). J Cell Biochem. 2007, 102(4):970-9.
41. Zheng Q, Chen J, Nie Z, Lv Z, Wang D, Zhang Y. Expression, purification and characterization of a three-domain Kazal-type inhibitor from silkworm pupae (Bombyx mori). Comp Biochem Physiol B Biochem Mol Biol. 2007, 146(2):234-40.
42. Huang F, Lv Z, Li Q, Wei L, Zhang L, Wu W. Study on hepatoprotective effect of peptide S-8300 from shark liver. World J Gastroenterol, 2005, 11(12):1809-12.
43. 吕正兵 主编. 生物工程制药学，北京，2012，科学出版社。
承担主要项目
1. 主持完成浙江省自然科学基金项目，鲨肝活性肽S-12000抗糖尿病研究；
2. 主持完成浙江省科技厅项目，马传染性贫血病毒减毒中的膜蛋白基因突变研究；
3. 主持完成横向课题“白藜芦醇体外生物合成研究”；
4. 主持横向重大项目“猪繁殖与呼吸综合征病毒基因工程疫苗的开发研究”；
5. 主持横向重大项目“昆虫抗菌肽生物合成及规模化制备研究”；
6. 主持横项目“重组大流行流感灭活疫苗（家蚕杆状病毒）样品试制及工艺探索研究”；
7. 校内主持国家重大新药创制项目子课题“新型Ⅰ型糖尿病口服药物的研制和中试“
8. 参与完成国家“863”重点项目课题，家蚕生物反应器蛋白质药物规模化制备技术，主持经费409万；
9. 参与完成国家科技支撑计划，家蚕生物反应器生产新型人用禽流感疫苗的试制；
10. 参与完成“973”项目子课题，家蚕小分子mRNA、小肽功能以及病原体与宿主相互作用机理研等。
